rabbit anti human a 2b (Alomone Labs)
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Alomone Labs
rabbit anti human a 2b
Rabbit Anti Human A 2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+human+a+2b/Anti-Adenosine+A2B+Receptor+(extracellular)+Antibody/pmc10997572-94-34-45
Average 93 stars, based on 20 article reviews
Rabbit Anti Human A 2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+human+a+2b/Anti-Adenosine+A2B+Receptor+(extracellular)+Antibody/pmc10997572-94-34-45
Average 93 stars, based on 20 article reviews
rabbit anti human a 2b - by Bioz Stars,
2026-09
93/100 stars
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Saline:Article Title: Blockage of the adenosine A 2B receptor prevents cardiac fibroblasts overgrowth in rats with pulmonary arterial hypertension Article Snippet: Equal amounts of protein per sample (150 μg/lane) were resolved by SDS-PAGE under reducing conditions (i.e., the samples were solubilized in SDS reducing buffer containing 0.125 mM Tris-HCl, 4% SDS, 0.004% bromophenol blue, 20% glycerol, and 10% 2-mercaptoethanol, pH 6.8 at 70 °C for 10 min) using 10% polyacrylamide gels, and electrotransferred onto PVDF membranes (Millipore, MA, USA). .. The membranes were blocked for 1 h in Tris-buffered saline (TBS: 10 mM Tris-HCl, pH 7.5, 150 mM NaCl) containing 0.05% Tween 20 + 5% BSA, and then probed overnight at 4 °C with Blocking Assay:Article Title: Blockage of the adenosine A 2B receptor prevents cardiac fibroblasts overgrowth in rats with pulmonary arterial hypertension Article Snippet: Equal amounts of protein per sample (150 μg/lane) were resolved by SDS-PAGE under reducing conditions (i.e., the samples were solubilized in SDS reducing buffer containing 0.125 mM Tris-HCl, 4% SDS, 0.004% bromophenol blue, 20% glycerol, and 10% 2-mercaptoethanol, pH 6.8 at 70 °C for 10 min) using 10% polyacrylamide gels, and electrotransferred onto PVDF membranes (Millipore, MA, USA). .. The membranes were blocked for 1 h in Tris-buffered saline (TBS: 10 mM Tris-HCl, pH 7.5, 150 mM NaCl) containing 0.05% Tween 20 + 5% BSA, and then probed overnight at 4 °C with |
![( A ) Immunohistochemical (IHC) staining to detect platelets <t>(CD41</t> + ) in healthy liver biopsies (Normal) and those from patients with AILI (Patient). Scale bar, 250 μm (n = 10/group). ( B ) Male C57B/6 mice treated with PBS or acetaminophen (APAP). Intravital microscopy analyses were performed around 3 hr post-APAP. Mɸs (cyan) and platelets (white) in liver sinusoids (red) are indicated. Representative images were chosen from intravital microscopy videos: https://bcm.box.com/s/15hmtryyrdl302mihrsm034ure87x4ea (Supplementary video 1, PBS treatment) and https://bcm.box.com/s/tuljfmstvv4lvoksx16fkxkpirkekynz (Supplementary video 2; n = 6–7 mice/group, 4–15 videos/mouse). ( C–E ) Male C57B/6 (wild-type [WT]) mice were treated with control IgG (Ctrl IgG) or an anti-CD41 antibody (α-CD41 Ab) either 3 hr before or 3 hr after APAP administration. ( C ) Serum levels of ALT and ( D ) liver histology with necrotic areas outlined were evaluated 24 hr after APAP treatment (n = 5 mice/group in C , D ). Scale bar, 250 μm. ( E ) Male C57B/6 (WT) and Chil1 -/- mice were treated with APAP. Additionally, Chil1 -/- mice were divided into two groups treated with either PBS or recombinant mouse Chi3l1 (rmChi3l1) simultaneously with APAP. Immunofluorescence (IF) staining was performed to detect intrahepatic platelets (CD41 + ) 3 hr after APAP treatment (n = 3 mice/group). Scale bar, 25 μm. Two-tailed, unpaired Student’s t-test was performed in A–C . One-way ANOVA were performed in E .](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3036/pmc08233036/pmc08233036__elife-68571-fig2.jpg)
![Schematic representation of the design of experiments on cultured enteric glial cells (EGCs). EGCs were treated for three days with palmitate (PA, 400 μM). Then, cells were incubated for 2 h with lipopolysaccharide (LPS, 10 μg/mL) before the addition of adenosine deaminase (ADA, 3 U/mL), to minimize interferences by extracellular adenosine. Under these conditions, cells were treated for 19 h with MRS1754 (0.1 µM, A <t>2B</t> <t>R</t> antagonist) or incubated for 1 h with MRS1754 before the addition of BAY60-6583 (0.05 µM, selective A 2B R agonist, 18 h). For the experiments with the inhibitor of glial cell-derived neurotrophic factor (GDNF) receptor signaling, cells were treated with RPI-1 (100 µM, 1,3-dihydro-5,6-dimethoxy-3-[(4-hydroxyphenyl)methylene]-H-indol-2-one) for 19 h. On the fourth day, cells were lysed for analysis of toll-like receptor 4 (TLR4) expression and the culture media were collected for analysis of substance P (SP), GDNF, and interleukin (IL)-1β release.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0602/pmc07290602/pmc07290602__cells-09-01245-g001.jpg)
![( A–C ) Chil1 -/- mice reconstituted with recombinant mouse Chi3l1 (rmChi3l1) were treated with either Ctrl IgG or α-CD44 Ab 30 min prior to acetaminophen (APAP) challenge. ( A ) Serum levels of ALT and ( B ) liver histology with necrotic areas outlined were evaluated 24 hr after APAP treatment (n = 4–5 mice/group). Scale bar, 250 μm. ( C ) Immunofluorescence (IF) staining was performed to detect intrahepatic platelets (CD41 + ) 3 hr after APAP treatment (n = 3 mice/group). Scale bar, 25 μm. ( D ) Flow cytometry analysis was performed to identify Chi3l1-binding cells among liver non-parenchymal cells (NPCs) isolated from wild-type (WT) mice treated with APAP for 2 hr. CD44 + cells were gated from single live cells. CD44 + cells that bind to rmChi3l1 were further gated. The Chi3l1 + CD44 + cells were then identified by markers for various cell types, including CD45 + CD146 - F4/80 + (Mɸs), CD45 - CD146 + (liver sinusoidal endothelial cells [LSECs]), and Ly6G + (neutrophils). Two-tailed, unpaired Student’s t-test was performed in A .](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3036/pmc08233036/pmc08233036__elife-68571-fig3-figsupp1.jpg)
